A little over a year ago, when the FSU Coastal & Marine Laboratory and WFSU-TV – a TV station – started this online enterprise, the understanding was that at some point this would end up being a show. And so here we are. As you may have gathered from that video up there, this will be about predators and prey: who’s eating whom, and who’s scaring whom. We will of course be doing this through the prism of David and Randall’s studies: the consumptive and non-consumptive effects of predators in salt marshes and oyster reefs, and the methods used to shine a light on these interactions. Continue reading →
Where did my winter of catching up on work go? And why is spring quickly hurtling into summer? YIKES!
…Okay, I feel better. All of us here feel a little behind on things, because this past winter and spring have been full of other projects (in addition to the oyster one) such as investigating how the oil spill affected marshes throughout the west coast of Florida and examining what all of those snails are up to out on Bay Mouth Bar. But now that summer is almost upon us, it’s time to move all hands on deck back towards the ambitious summer oyster goals.
Environmental vs. Predator Effects.
To lay the ground work for this summer’s oyster research, I spent a few days in St. Augustine, Florida, which is where we will conduct our colossal field experiment. As a recap of the oyster objectives, we spent year 1 monitoring the oyster food web at 12 estuaries between Florida to North Carolina. Well, we found some cool patterns regarding the food web and water-filtration/ nutrient cycling services on oyster reefs (see the 2010 wrap-up). So, now we want to know what’s causing those patterns. Are differences in oyster reefs between NC to FL due purely to differences in water temperature, salinity, or food for oysters (phytoplankton)? Or, do we have a higher diversity of predators down south that are exerting more “top-down” pressure on the southern reefs? Or, is it a combination of the environment and predators? Continue reading →
David's collaborators, from left to right- Dr. Jeb Byers, Dr. Mike Piehler, Dr. Jon Grabowski, and Dr. Randall Hughes.
As you can see from the video that summarized our efforts over 2010, it was a busy 6 months of research. After taking a great break during the holidays, the entire oyster team (Jon = Gulf of Maine Research Institute, Mike = University of North Carolina at Chapel Hill, Jeb = University of Georgia, Randall = Florida State University and me) met for a long weekend to figure out what we accomplished and where we are going in the future.
You might think that our 2011 research plans should already be set given that we received funding. Well, we did receive funding to carry out some outlandish field experiments in 2011, but these experiments were dreamed up in our offices and may not address the most ecologically relevant questions for our system. Checking in with the monitoring data is probably the best way to determine if our planned experiments were on target or if they needed to be adjusted and hopefully simplified!
Prior to the oyster summit last weekend, I hounded all of the research teams for all of their data. Given the huge volume of data and everyone’s busy schedules with teaching classes and other research projects, this was quite the task. Once Tanya meshed all the data together (also not a simple task), I then moved on to the next task of analyzing our data.
Well, the initial excitement quickly turned into a stomach churning feeling of….where the heck do I begin? Similar to the way that too many prey can reduce the effectiveness of predators, the data were swamping me…I was overwhelmed and the draining hourglass wasn’t helping (people were flying into town in two days…yikes!).
After multiple cups of coffee, the anxiety passed and I decided to revisit some basic questions:
David's team used gill nets to catch the larger fish around the reefs, many of which are top predators in that habitat.
(1) With the gill nets, we obtained predatory fish data. So how do the abundance and biomass of these fishes vary across latitude? And does this pattern change with season (i.e., summer versus fall)?
(2) Then I thought back to the fond memories of ripping up oyster habitat to check out the abundance of things that consume oysters (e.g., mud crabs). Oh…the memory of that work gives me a warm and fuzzy feeling; I bet Tanya, Hanna, Linda and everyone else that helped feel the same way! How do the abundances of these things change across latitude? Are there larger crabs up north or down south? How does the mud crab picture mesh with the predatory fish picture?
This spat stick is made of calcium carbonate, the same substance as oyster shell, and is ridged to simulate the ridges in those shells. That makes it an attractive landing spot for oyster spat (larval oysters), which tend to settle on oyster shells.
(3) Working our way down the food web and sticking with the oyster samples we ripped up back in August, how do oyster densities and oyster size change across latitude and how do these patterns mesh with the mudcrab and predatory fish data?
(4) Finally, I wanted to revisit the data from our instrumentation to see how temperature and salinity changed across latitude and with season, as well as the data from our spat sticks to see how oyster recruitment differed.
It’s pretty amazing that six months of work can be summarized so quickly into four topics. Well, I kept hitting the coffee and got all of these data worked up in time for the first portion of our oyster summit. Surprisingly, all inbound flights arrived on time and we all assembled last Friday to go over the data. I’ll briefly lift the research curtain to illustrate what our data looked like:
The Georgia reef gill nets trapped a lot of sharks. Here Dr. Jeb Byers is removing blue crabs (also an oyster reef predator) from shark bellies. The trapping done on these reefs is clarifying the food web for these habitats.
(1) Although we predicted predator abundance to increase at lower latitudes, predator abundance and the number of different predators peaked in Georgia/South Carolina. This is because lots of the species we have in Florida were also in Georgia. And, Georgia has lots of sharks! Needless to say, Jeb’s crew has been the busiest during gillnet sampling. Jon and Mike’s crew have had it pretty easy (no offense)! The workload reduced for everyone in the fall, but the differences across latitude stayed relatively the same. The really cool result was the pattern that hardhead catfish are extremely important and the most abundant predatory fish on Florida reefs; I love those slimy things.
(2) Interestingly, mudcrab biomass peaked up north where predatory fishes were less abundant.
(3) And the abundance of large, market size oysters was highest where predatory fish were most abundant (GA/SC).
(4) Amazingly, we all did a good job selecting oyster reefs with equivalent salinities (this can vary a lot just within one estuary) and temperature was the same across all of our sites until December….instrumentation up north got covered in ice! Glad I was assigned the relatively tropical reefs in Florida. Finally, oyster recruitment in NC and Florida appears to proceed at a trickle while that of GA/SC is a flood-like situation during the summer.
A month after first being deployed, Tanya and Hanna inspect an Alligator Harbor tile. You can see that some of the oysters have definitely started growing, but also that some of the spat became unglued. When they run the experiment again, they'll use a different adhesive more suitable for a marine environment.
After we all soaked that in, we then talked about the tile experiment. While these data were really cool (mortality presumably due to mudcrabs was lowest where predatory fish were most abundant = GA), we worried about being able to tease apart the effects of flow, sedimentation, and predation. Unfortunately, this experiment seems to uphold my record with experiments: they never work the first time. We’ll probably repeat this in fall of 2011 with a much better design to account for flow and sedimentation.
Before breaking for a nice communal dinner at my place, Mike summarized the nutrient cycling (sediment) data that we have been collecting. In short, having lots of living oysters really promotes de-nitrification processes and our sampling picked this up.
Putting this all together, it looks like there are latitudinal patterns in fish predators that may result in mudcrab density and size patterns. Together, these may help account for latitudinal patterns in oysters (highest in GA). This all matters because more oysters = more denitrification = healthier estuarine waters.
END DAY 1
On day 2 of the summit, we worked through what made us happy about the monitoring data, what things we could add on to make us happier, and that we should continue this monitoring through the summer of 2011. This actually took all morning.
On day 2, the oyster summit moved into the more comfortable location of the Marine Lab guest house.
After a quick lunch break, we then reconvened in another room with a better view (nice to change up the scenery) to go over how we should experimentally test the linkages I mentioned above. This is where the saw blade of productivity met a strong wood knot. Personally, I became horribly confused, fatigued and was utterly useless. This resulted in lots of disagreement on how to proceed and possibly a few ruffled feathers. But nothing that some good food and NFL playoff football couldn’t cure.
After taking in a beautiful winter sunset over the waters off the lab, we ditched the work and began rehashing old and funny stories about each other.
Amazingly, we awoke the next morning and fashioned together a great experimental design that we will implement beginning June 2011. To Jeb’s disappointment, this will not involve large sharks, but we will get to play with catfish!
But now it’s time to prepare for our winter fish and crab sampling. It will be interesting to see what uses these reefs during the dark and cold of winter!
Thanks for following us during 2010, and please stick around for 2011 as I’m sure things will get really interesting as we prepare for our large field experiment.
David’s research is funded by the National Science Foundation.
Along with David’s remembrances of his early life in marine biology, we have a video on one of David’s collaborators in this oyster study, Jeb Byers. Like all of the collaborators on the study, Jeb attended the University of North Carolina, where he overlapped with Jon Grabowski. Alicia Brown was sent up to help Jeb’s team during the October Oyster Push, so we lent her a Flip camera to document the proceedings. She got footage of some of the fish they caught, including the sharks that predate their reefs.
Dr. David KimbroFSU Coastal & Marine Lab
L to R- Tanya Rogers, Dr. Jon Grabowski, Hanna Garland, and Dr. David Kimbro. Here you have three "generations" of researchers and techs. Just as David was once Jon's lab technician, Hanna and Tanya help David today with his projects.
Burrrrr….it’s cold down here and I love it…a nice break from the no see’ums! We are gearing up to hit the road for some regular sampling (water/sediment sampling and down load instrumentation) as well as to check on the tile experiment that began 6 weeks ago. Props again to Tanya for getting us organized to go! Although, I have some anxiety about what I’ll see on the tiles because the adhesive we used to affix the oysters may not be working as planned; more on that that in the next post after we get a visual on things.
For now, I want to pick up where Randall last left off by reminiscing about how I first got into the research/oyster business and how it’s all Jon’s fault. Like Randall, I graduated from the University of North Carolina at Chapel Hill and was equally clueless about what I wanted to do in life. However, I did know that the coast was where I wanted to be.
While Randall, Jon, and many others where schlepping around tons of oyster shell in the hot North Carolinian summer, I was having a good time surfing by day and waiting tables by night. All in all, I’d say that my summer was much more relaxing than theirs!
But after spending lots of time enjoying the coastal environment, I realized that I needed to look into this whole marine science thing. So, I began to nose around UNC’s marine lab and volunteered a little bit. By this time, Randall had taken off to teach middle school and Jon just got a prestigious offer to conduct research in Antarctica. But there was one glitch: who was going to run his oyster project in NC? He couldn’t just push the pause button on this research. Luckily, he had one last greater helper (Meg) whom he began training to be the boss. But she needed an underling. Enter me. Because they could not find a qualified research technician within three counties to hire, Jon decided to give ignorant me a shot. I was immediately told that the work was grueling and that the pay was peanuts. But I figured it had to be better than sitting indoors and watching the clock. Plus, Randall had already done the hard work by building all of those reefs; thank goodness I wasn’t on board for that madness!
Reaping the rewards from all the hard work that Randall and Jon exerted to build the oyster reefs, I got the easy work of just monitoring them and it was fun. When Jon returned from Antarctica, he saw that I hadn’t messed up anything too badly. That, coupled with my always asking him research questions made him decide to give me a little project of my own. And it is this experience that really sent me on my way into marine ecology. So, as I paddle my kayak out to the oyster reefs, think about interesting research questions, and enjoy the scenery, I often think back about the wonderful and fortuitous opportunity that Jon first gave me.
Mud crab (Panopeus herbstrii)
Ok, do I have any stories? Of course. One classic story that seems to get re-told every time Jon and I get together concerns our ripping up his restored oyster reefs to see what critters lived within them. Now, Jon was really interested in mud crabs, how they affected oysters by eating them, and how larger predators affected this dynamic by eating or scaring the mud crabs. So, while I (the rookie) was working through samples, he was a bit concerned that I was missing many of the smaller crabs. Knowing about his concern as well as being a little bit grumpy about being over worked and being a little naughty, I decided to leave about 5 or so pretty large mud crabs in my sieve. I then said, “hey Jon, to make sure I’m doing this correctly, will you check over my sample to see if I missed any crabs?”. By this time, I had already processed many, many hours worth of samples. So, when Jon looked at my sieve, he immediately freaked out and thought about how many of the other samples I must of messed up. Oh, I had such a good laugh. Thirteen years later, I think this story still gets Jon’s blood pressure up.
Years later, David heads his own team, and he and Randall are colleagues and collaborators with Dr. Grabowski.
What else…well, the winter work was so boring in North Carolina (lots of indoor time spent going through sediment samples) that I had to turn to coffee to help me make it through the late afternoon; with Meg’s persuasion (she was an addict and wanted some company). I stubbornly refused this drug all throughout college because I did not want to be an addict with smelly coffee breathe. But Meg was very persuasive and she started me out with small doses of Dunkin Donuts froofy, flavored coffees. Boy, this and some good 80’s music really helped me survive the late afternoon hours of sorting Jon’s samples in the lab. Next thing you know, I’m asking Jon for a coffee break (“hey man, can I take a quick trip to the Double D?”) every afternoon. Because Jon was a stingy boss (I say this with love), my and Meg’s new afternoon routine really annoyed Jon. But gosh, had I been open-minded about the joys of coffee back in college, I would have graduated with honors! In summary, the boringness of Jon’s project during the winter gave rise to my love of coffee (as Tanya eloquently captured in her last post), and it bugged the crap out of Jon…that and my caffeinated singing of 80’s songs in his lab during the later winter afternoons.
I could keep going with more stories, but I don’t want to give Tanya and Hanna any ideas or ammunition, so I’ll stop here.
David’s research is funded by the National Science Foundation.
The following is the first of three or so videos on the big October oyster trip. In this one, you get a long busy day in the field condensed into two minutes (it’s much less exhausting that way). We’ll have videos in the next couple of weeks on David’s co-collaborators (including video of the Georgia/ S. Carolina team and all the sharks they caught) and a video on David’s own team.
The "October Oyster Push" had many objectives, but none took as much time to implement than the tile experiment. Seeing how these baby oysters- spat- grow over the next few months will give David an idea how oysters typically fare at each reef over the course of their lives.
I spent most of this past week feeling pretty darn good about having just finished our October sampling and experimental objectives out on the oyster reefs. Of course, this glow continued into the weekend as my football team pulled out a W in Tallahassee.
But back to the science. Although Rob chronicled each day of our crazy road trip, I want to relive it once more just to give the trip from my perspective. So, here are my top-ten thoughts:
Number 1: Planning the details of the road trip (housing, which team is going where and when) as well as figuring out how to set up the tile experiment (see video) was pretty stressful. Thank goodness I had Tanya around to bounce scheduling ideas off of. Because I kept chaning my mind, I think Tanya made like 6 different versions of our schedule.
Number 2: I talked the NC and SC/GA teams into doing the aforementioned experiment with oyster spat to examine how actual predation and the fear of being eaten affects oysters up and down the coast. I successfully convinced the teams partly because I emphatically claimed that the additional work load would only be five hours of more work at each site. Well, I got that wrong. It was probably triple that estimate. That’s one of my flaws: I always underestimate how long research tasks take, which is bad because you constantly feel behind as a result of being over-scheduling. Rule of thumb: always multiply my work estimates by at least 2.
Number 3: I never want to see a dremel again. With dremel in hand one evening at Saint Augustine, I had only extracted ¼ the spat I needed for the experiment but the time spent on this task had already surpassed my previous estimate. That’s when coffee and the ability to lose yourself in the task become extremely important. I guess I took it one oyster spat at a time.
(L to R) Tanya, Hanna, and Cristina pick up the slack while David dremels away back at the lab space.
Number 4: I could not have lost myself in the task of setting up the experiment if it hadn’t been for Tanya, Hanna and Cristina. Knowing that they were fully trained to carry out the sampling objectives, I did not have to busy myself with those numerous tasks, such as setting gill nets and traps (and retrieving the catch), taking sediment and water samples, etc. In fact, after finishing the sampling objectives and follow-up lab work, they would immediately begin helping me with the experiment by cleaning adult oysters and identifying spat for me to extract with the dremel. With that help, I was able to focus solely on dremeling.
Number 5: Dremeling 1080 spat out of adult oyster shell stinks. Did I already say that? Well, this task deserves two spots on the top-ten list. In tact, I probably attempted to extract over 2,000 oysters because I would often slip with the dremel and accidentally kill the oyster spat that I had spent five or so minutes on.
Hardhead and sail catfish seem to be the dominant predator of the Florida Gulf sites. By eating mud crabs that predate oysters, these fish perform an important function on oyster reefs.
Number 6: we couldn’t have asked for better weather. In fact, I think there were some temperature records being set. Despite these warmer than usual temperatures, there was about ½ the diversity and number of predatory fish on our reefs. So, going against my expectations, these Florida sites are experiencing some seasonality in the assemblage of predators. Interestingly, all teams were catching red drum on their reefs; guess it’s their time of year. The red drum mostly had smaller fish in their stomachs. The SC/GA team was still catching lots of sharks. And catfish was still the most abundant predator on our reefs. Those slimy things are definitely major players on southern oyster reefs because they had lost of mud crabs (who eat oysters) in their guts. Final detail about the Florida sites is that my northern locations (Alligator Harbor on Gulf and Jacksonville area on Atlantic) had more predatory fishes than did the more southern sites in Florida…. intriguing.
Number 7: We had to change plans at the end of the week and this mid-course change actually went smoothly. This change came about because the housing space near our Jacksonville site was not conducive for setting up the tile experiment. Luckily, Hanna and Cristina ventured up to Jacksonville to figure all of this out for me. This “divide and conquer” strategy allowed Tanya and me to finish up the sampling and experimental objectives in Saint Augustine, while Hanna and Cristina began sampling in Jacksonville to keep us on schedule. And rather than resting up in Jacksonville, Hanna and Cristina ripped up oyster habitat and drove it back down to Saint Augustine. They looked pretty rough upon that later return to Jacksonville. But after a good dinner and a few hours of sleep, their oyster delivery allowed us to work on the materials for the Jacksonville experiment in a much better laboratory setting.
Number 8: Team morale and will to finish objectives hit a low point once we reached Jacksonville. The lodging for the first evening was haunted with cockroaches: this is Hanna’s kryptonite. Luckily, Tanya whipped us up some good pasta to help keep our minds off of the roaches. The next morning, cockroaches began to seem not so bad. When we got to the boat-launch and found there to be no wind, I knew it was trouble because this site had the reputation for being particularly buggy. So, we headed into the mouth of our creek and hit the first reef. Not too bad… actually, no fish in the nets. Only a few bugs and two free hands to swipe them away. But as we ventured further into the belly of the creek/bug hell and found tons of fish in our nets, I began to worry about mutiny. As I was exhorting the crew to extract tons of fish from the next set of nets, I realized that freeing this many fish would take twice as long because we needed to spend an equal amount of time cursing the no-see’ums and keep them out of our ears and noses; kind of hard to do with fish in your hands. While taking fire from the no-see’ums, we then began sustaining additional injuries from other natural agents. I suffered my first good-sized oyster cut. Hanna got her finger nearly cut off by a large stone crab. For the pain finale, a decent sized catfish stabbed my hand with the barb of its dorsal fin. I don’t blame it, but daggum that hurt. At this point, the unpleasantness was almost comical. Note to self: buy hats with bug nets to combat no-see’ums.
Number 9: All of the pain and stress of that week is now good fodder for the lab to laugh about and bond over. That’s one of the perks of conducting research as a team. And that’s one of the reasons why Big Jon, Randall and I are still collaborating.
David walks away from the tiles he and his team spent so much time putting together. He won't know how successful the experiment was until he travels back to these sites.
Number 10: Now that we have all caught up on sleep, have relived our stories, and have begun to look at the data, I now stress about whether the tile experiment will actually work. Like most experiments I conduct, I put a lot of effort into something that has a 50% chance of not succeeding. For example, the spat that I extracted and adhered to tiles may have been overheated by the dremel/extraction process…are they dead already? And then, oh boy…what if the glue doesn’t hold? That’s what really keeps me up at night.